Evaluation of rs1957106 Polymorphism of NF-kappaBI in Glioblastoma Multiforme in Isfahan, Iran

Abstract

Background: The kB family of nuclear factor (NF-kappaB) is a series of transcription factors that plays a key role in regulation of immunity, cell growth, and apoptosis and is considered as the main downstream component of epidermal growth factor receptor for which there are evidence of excessive activity in most cases of glioblastoma multiform (GBM). Thus, the current information has gained evidence on NF-kappaBIA tumor suppressor role in GMB. SNP rs1957106 was diagnosed as a new polymorphism which affected the expression of NF-kappaBI and causes activation of NF-kappaB in GBM patients. Materials and Methods: This study was conducted on 100 cases of GBM including 47 paraffin-embedded brain tissue samples and 53 blood samples from another 53 GBM patients and 150 controls. The NF-kappaBI rs1957106 SNP was identified by the NCBI, and genotyping was performed by high-resolution melt (HRM) assay. Melt curves from HRM which suspected to single-nucleotide polymorphism (SNP) were selected and subjected to direct sequencing. Results: The distribution of allele A of NF-kappabeta gene in patients with GBM with 31 was not significantly different from healthy participants (27.3) (P = 0.375). Furthermore, the distribution of AG and GG genotypes in comparison with AA genotypes did not show a significant correlation with GBM incidence (P > 0.05). Conclusion: Findings of the present study provide evidence that the rs1957106 SNP in NF-kappaBIA is found more in GBM patients, but it was not statistically significant. As there are conflicting studies showing significant higher rate of this SNP in GBM, further study is suggested.