The expression of human leukocyte antigen by human ejaculated spermatozoa

(2019) The expression of human leukocyte antigen by human ejaculated spermatozoa. Molecular Genetics & Genomic Medicine. p. 8. ISSN 2324-9269

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Official URL: WOS:000490817100001

Abstract

Background After coitus and insemination, an inflammatory response is evident in the female reproductive tract (FRT). Semen contains a variety of immune-activating components that have a major role in the induction of an immune response in the FRT. One of the most important is (human leukocyte antigen) HLA molecules which are present in soluble form in seminal plasma and in membrane form on the surface of cells (such as epithelial and leukocytes) existing in semen. Nevertheless, there is considerable debate over the expression of HLA antigens by human spermatozoa. Considering the critical role of HLA molecules in reproduction and the induction of an immune response, it is very important to clearly define HLA expression by spermatozoa and the role of these molecules in sperm morphology, motility, and strength to fertilize an egg. Therefore, the objective of this study was to determine HLA expression by ejaculated spermatozoa. The results of this study will facilitate the design of future studies. Method Semen samples were collected from 50 healthy men with normal semen status by masturbation after 2-3 days of sexual abstinence. After purification of normal spermatozoa, HLA class I & II expression was evaluated by quantitative real-time PCR and flow cytometry methods. Results The results showed the expression of both HLA class I & class II by spermatozoa. The results also showed that the expression of HLA class II was significantly more than HLA class ROMAN NUMERAL ONE. Conclusion Spermatozoa express both HLA class I & class II molecules.

Item Type: Article
Keywords: HLA-Ia HLA-II spermatozoa hla-c genes class-i seminal fluid maternal kir sperm cells molecules transcripts pregnancy responses failure Genetics & Heredity
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 100-133 Biochemistry of the Human Body
WJ Urogenital System > WJ 700-875 Male Genitalia
Divisions: Faculty of Medicine > Department of Basic Science > Immunology Department
Faculty of Medicine > Departments of Clinical Sciences > Department of Obstetrics and Gynecology
Page Range: p. 8
Journal or Publication Title: Molecular Genetics & Genomic Medicine
Journal Index: ISI
Volume: 7
Number: 12
Identification Number: https://doi.org/10.1002/mgg3.1005
ISSN: 2324-9269
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/11412

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