(2020) Protective effects of zinc on rat sperm chromatin integrity involvement: DNA methylation, DNA fragmentation, ubiquitination and protamination after bleomycin etoposide and cis-platin treatment. Theriogenology. pp. 177-183. ISSN 1879-3231 (Electronic) 0093-691X (Linking)
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Abstract
Testicular cancer is one of the most common malignancy in young men, chemotherapy induced damage in cancerous cells as well as healthy tissue, and we decided to investigate recovery effect of zinc (Zn) on chemotherapy-induced complications in rat chromatin integrity and testicular histomorphometry. The male rats (n=40) were treated with BEP at appropriate dose levels of BEP (0.75, 7.5, and 1.5mg/kg) for 9 weeks, with or without Zn; testicular histology, sperm DNA methylation, ubiquitination, DNA fragmentation and protamination were further assessed through immunofluorescence. BEP treatment significantly increased ubiquitination, and DNA fragmentation, considerably reducing global DNA methylation and protamination (P<0.001), resulting in degenerative changes in testicular structure. Zn restored normal DNA methylation, protamination and structure of male gonads, maintained spermatogonial stem cells, and significantly reduced the mean percentage of ubiquitination and sperm DNA fragmentation as compared with BEP group (P<0.001). We found that supplementation of Zn following chemotherapy can improve chromatin integrity, testicular organization and spermatogenesis.
| Item Type: | Article |
|---|---|
| Keywords: | Animals Antineoplastic Combined Chemotherapy Protocols/*adverse effects/therapeutic use Bleomycin/administration & dosage/adverse effects Chromatin/*drug effects/metabolism Cisplatin/administration & dosage/adverse effects Cytoprotection/*drug effects/genetics DNA Fragmentation/*drug effects Etoposide/administration & dosage/adverse effects Fertility Preservation/methods Genomic Instability/drug effects Infertility, Male/chemically induced/prevention & control Male Neoplasms, Germ Cell and Embryonal/drug therapy/pathology Protamines/metabolism Protein Processing, Post-Translational/*drug effects Rats Rats, Wistar Spermatozoa/*drug effects/metabolism Testicular Neoplasms/drug therapy/pathology Ubiquitination/drug effects Zinc/*pharmacology/therapeutic use Anticancer drugs combination DNA damage DNA methylation Protamination Spermatozoa Ubiquitination |
| Subjects: | QZ Pathology > QZ 200-380 Neoplasms WJ Urogenital System > WJ 700-875 Male Genitalia |
| Divisions: | Faculty of Medicine > Department of Basic Science > Department of Anatomical Sciences |
| Page Range: | pp. 177-183 |
| Journal or Publication Title: | Theriogenology |
| Journal Index: | Pubmed, ISI |
| Volume: | 142 |
| Identification Number: | https://doi.org/10.1016/j.theriogenology.2019.09.039 |
| ISSN: | 1879-3231 (Electronic) 0093-691X (Linking) |
| Depositing User: | Zahra Otroj |
| URI: | http://eprints.mui.ac.ir/id/eprint/11645 |
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