Molecular analysis of Coxiella Burnetii by isocitrate dehydrogenase gene sequence-based typing and PCR-RFLP in Isfahan, Iran

Abstract

In the recent years, considerable advances have been made in the detection and genotyping of Coxiella burnetii, the causative agent of Q fever. The selection of appropriate genotyping method has enabled description of the clonal diversity of C. burnetii around the word. Since, in the place of study, C. burnetii genotyping has not been done, the icd gene Restriction fragment length polymorphism (RFLP) and sequence-based typing for differentiation between the genomic detected C. burnetii from the various sources and compared the two methods is used. In a observational study, a total of 15 genomic positive cases of C. burnetii infection from different sources in Isfahan province (Central Iran) were enrolled and underwent two genotyping methods: the icd gene PCR-RFLP and icd gene sequence-based typing. The degree of similarity between the icd gene sequences was high (98.3-100). In compare with C. burnetii Nine Mile icd gene sequence, the nucleotide sequences were different at 11 positions, which resulted in 7 differences in the amino acid sequences. After digesting the 370 bp amplified icd gene fragments all the samples indicated only one band of 370bp, while amplified C. burnetii Nine Mile strain icd gene were digested into two bands with sizes of 221bp and 149bp. The results of two genotyping methods matched together. Used methods in present study were cheaper and easier than new methods and they can used for detection of acute and chronic phases of infection. © Scienceline Publication.