Effect of cleaning guidelines implementation on microbial colony count of laparoscopic instruments: A study in a public hospital in Iran

(2020) Effect of cleaning guidelines implementation on microbial colony count of laparoscopic instruments: A study in a public hospital in Iran. Asian Journal of Endoscopic Surgery. pp. 272-278. ISSN 1758-5902

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Abstract

Introduction Some countries have implemented reuse of laparoscopic instruments for cost-effective purposes. An accurate cleaning as the first step of reprocessing would lead to the effective sterilization. The purpose was to evaluate the effect of cleaning guidelines implementation on microbial load of laparoscopic instruments which were used in laparoscopic cholecystectomy surgery. Methods This experimental study was done in an educational hospital, in 2017 and included a total of 128 laparoscopic instruments randomly selected from cholecystectomy surgeries and divided into two cleaning groups. The instruments were checked out in terms of number (colony-forming units CFU/mL) and type of microorganisms in two groups of routine cleaning and according to guideline cleaning. This guideline was indigenous and taken from successful instruction in this context that was presented by the Association for the Advancement of Medical Instrumentation (AAMI). The appropriate statistical analysis was conducted by SPSS version 19. Results The average microbial load was 2.4 x 10(6) CFU/100 mL after clinical use. It was reduced to 7.2 x 10(5) CFU/100 mL in the control group and 3.4 x 10(4) CFU/100 mL in the intervention group, after the cleaning process. The most common microorganisms that were isolated immediately after clinical use were Escherichia coli 81.2%, Pseudomonas 68.8%, Klebsiella 57.8%, and spp., and so on. Conclusion The AAMI cleaning method is recommended to be utilized by operating room nurses for laparoscopic instruments.

Item Type: Article
Keywords: cleaning laparoscopy microbial colony count surgical instruments SURGICAL-INSTRUMENTS CLINICAL-USE LOAD CONTAMINATION DISINFECTION INFECTIONS SURGERY
Subjects: QW Microbiology and Immunology > QW 1-300 Microbiology
WO Surgery
Divisions: Faculty of Medicine > Department of Basic Science > Department of Microbiology
Other
Page Range: pp. 272-278
Journal or Publication Title: Asian Journal of Endoscopic Surgery
Journal Index: ISI
Volume: 13
Number: 3
Identification Number: https://doi.org/10.1111/ases.12750
ISSN: 1758-5902
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/12003

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