Preparation and in vivo evaluation of a novel gel-based wound dressing using arginine-alginate surface-modified chitosan nanofibers

Abstract

The development of an effective wound dressing with the ability to induce skin wound healing is a great challenge in medicine. Nanofibers are highly attractive for wound dressing preparation due to their properties such as hemostasis induction, good absorption of wound exudates, and facilitation of cell growth. Chitosan nanofibers have attracted great attention for application in wound dressings due to their accelerating effects on wound healing. In this study, arginine surface-modified chitosan nanofibers were successfully prepared by attachment of arginine molecules on the surface of chitosan nanofibers using sodium alginate through electrostatic interaction. The effect of pH on the amount of attached arginine was evaluated at three different pH values; 5, 6, and 7. Fourier-transform infrared spectroscopy and zeta potential of chitosan nanofibers before and after surface modification suggested the occurrence of the attachment of arginine to chitosan nanofibers. Scanning electron microscope images showed the nanofibrous structure of arginine surface-modified chitosan nanofibers with an average diameter ranging from 100 nm to 150 nm. The release of arginine from arginine surface-modified chitosan nanofibers gel showed a sustained release manner. The suitable viscosity and spreadability of arginine surface-modified chitosan nanofibers gel verified its easy application at the wound site. Arginine surface-modified chitosan nanofibers gel significantly improved the wound healing process including wound closure when tested in vivo using rat model. Additionally, histological examination and immunohistochemical studies showed the significant enhancement of the re-epithelialization, collagen deposition, and angiogenesis in the skin of the animal group treated with arginine surface-modified chitosan nanofibers gel compared with the other control groups. These results suggested that arginine surface-modified chitosan nanofibers gel could be introduced as an effective wound dressing.