Development of High Resolution Melting Analysis as a Diagnostic Tool for Molecular Detection of Toxoplasma Infection in Pregnant Women and HIV Positive Cases

(2020) Development of High Resolution Melting Analysis as a Diagnostic Tool for Molecular Detection of Toxoplasma Infection in Pregnant Women and HIV Positive Cases. Iranian Journal of Public Health. pp. 1983-1991. ISSN 2251-6085

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Abstract

Background: Toxoplasma gondii is an obligate intracellular protozoan with worldwide distribution. Diagnosis of toxoplasmosis is a very critical issue, especially in pregnant women and immunocompromised patients. The aim of this study was rapid detection of T. gondii DNA in peripheral blood samples (PBS) employing HRM technique and using RE gene. Methods: Totally, 242 samples from pregnant women and human immunodeficiency virus (HIV) patients were collected from different hospitals and medical centers of Tehran during Oct 2017 to Dec 2018. High resolution melting analysis (HRM) using partial sequences of repetitive element (RE) gene was done and compared with ELISA test. Results: Overall, 51 were positive for acute toxoplasmosis that among them, 12 and 20 reported as positive in pregnant women and HIV+ patients, respectively using HRM technique. Among 70 patients in chronic phase of disease, 10 and 3 samples were reported as positive for pregnant women and HIV+ patients respectively. From 121 negative control, 3 (4.62) samples associated with HIV+ patients, showed positive real-time PCR and HRM analysis results. Conclusion: For the first time, HRM technique via employing RE gene was used for detection of T. gondii infection in PBS. This method is suitable, helpful and in parallel with serological methods for early diagnosis of acute as well as active form of toxoplasmosis in pregnant women and HIV+ patients. The use of techniques based on melt curve and through employing next-generation dyes for diagnosis of T. gondii would be accessible for patients in developing countries.

Item Type: Article
Keywords: High-resolution melting (HRM) Toxoplasmosis Pregnant women HIV+ patients REAL-TIME PCR POLYMERASE-CHAIN-REACTION ACQUIRED TOXOPLASMOSIS GONDII INFECTION IDENTIFICATION SAMPLES TEHRAN ELISA GENE
Subjects: WQ Obstetrics > WQ 200-212 Reproduction. Pregnancy
WR Dermatology > WR 140-340 Skin Diseases
Divisions: Skin Diseases and Leishmaniasis Research Center
Page Range: pp. 1983-1991
Journal or Publication Title: Iranian Journal of Public Health
Journal Index: ISI
Volume: 49
Number: 10
ISSN: 2251-6085
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/12774

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