(2020) Identification of an Immunoglobulin M (IgM) Antibody Against Enolase 1 Protein (ENO1) Derived from HEK-293 Cells in Patients with Kidney Failure. International Journal of Peptide Research and Therapeutics. pp. 1251-1257. ISSN 1573-3149
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Abstract
Kidney failure is one of the most important challenges in medicine. In this study, we used HEK-293 kidney cells to evaluate and compare changes in the expression of natural antibodies in patients with kidney failure and healthy controls. We analyzed the immunoreactivity of two groups of pooled sera from patient and control groups against antigens derived from HEK-293 cell lysates. The cell lysates were immunoblotted with the pooled sera from the groups. The results showed that the patients' sera contained IgM antibodies, which recognized enolase 1 proteins. Graphic
| Item Type: | Article |
|---|---|
| Keywords: | Kidney failure HEK-293 cell line Enolase 1 Two-dimensional electrophoresis PROTEOMICS-BASED APPROACH URINARY PROTEOMICS INJURY BIOMARKERS DISEASE EPIDEMIOLOGY CANCER |
| Subjects: | WJ Urogenital System > WJ 300-378 Kidney |
| Divisions: | Cardiovascular Research Institute > Applied Physiology Research Center |
| Page Range: | pp. 1251-1257 |
| Journal or Publication Title: | International Journal of Peptide Research and Therapeutics |
| Journal Index: | ISI |
| Volume: | 26 |
| Number: | 3 |
| Identification Number: | https://doi.org/10.1007/s10989-019-09919-y |
| ISSN: | 1573-3149 |
| Depositing User: | Zahra Otroj |
| URI: | http://eprints.mui.ac.ir/id/eprint/12859 |
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