LNA Inhibitor in microRNA miR-23b as a Potential Anti-proliferative Option in Human Hepatocellular Carcinoma

(2020) LNA Inhibitor in microRNA miR-23b as a Potential Anti-proliferative Option in Human Hepatocellular Carcinoma. Journal of Gastrointestinal Cancer. pp. 109-115. ISSN 1941-6628

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Abstract

Purpose Dysregulation of microRNAs (miRNAs) has been shown to be involved in the pathogenesis and progression of many malignancies. Human hepatocellular carcinoma (HCC) is one of the most common cancers worldwide and the third cause of cancer-related deaths. Recent data suggest that microRNA-23b (miR-23b) is significantly high in different types of cancer, specifically human hepatocellular carcinoma. Locked nucleic acid (LNA)-modified oligonucleotides have recently been suggested as a novel approach for targeting miRNAs as antisense-based gene silencing. The aim of this study was to explore the functional role of LNA-anti-miR-23b in a HepG2 (hepatocarcinoma) cell line. Methods HepG2 cells were transfected with LNA-anti-miR-23b for 24, 48, and 72 h. Quantitative real-time reverse transcriptase-PCR (qRT-PCR) was performed to assess miR-23b expression by LNA-anti-miR-23b. The viability of the cells was evaluated by MTT (3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay. Results LNA-anti-miR-23b was successfully transfected into human HepG2 cells and suppressed the miR-23b. LNA-anti-miR-23b reduced the invasive behaviors of HepG2 cells after 24 h, compared to untreated cells and scrambled LNA-transfected cells, and this effect was more pronounced after 72 h. Conclusions Our findings suggest that inhibition of miR-23b could be used as a novel approach in inhibition of HCC proliferation.

Item Type: Article
Keywords: microRNA miR-23b Human hepatocellular carcinoma Locked nucleic acid CANCER MIRNAS DEGRADATION METASTASIS PROTEIN
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
QZ Pathology > QZ 200-380 Neoplasms
WI Digestive System > WI 700-770 Liver. Biliary Tract
Divisions: Faculty of Medicine > Department of Basic Science > Department of Molecular Medicine and Genetics
Page Range: pp. 109-115
Journal or Publication Title: Journal of Gastrointestinal Cancer
Journal Index: ISI
Volume: 51
Number: 1
Identification Number: https://doi.org/10.1007/s12029-019-00215-y
ISSN: 1941-6628
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/13318

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