Releasing and structural/mechanical properties of nano-particle/Punica granatum (Pomegranate) in poly(lactic-co-glycolic) acid/fibrin as nano-composite scaffold

Abstract

The effect of poly(lactic-co-glycolic acid) (PLGA) on structure, degradation, drug release and mechanical properties of fibrin/pomegranate(F/POM)-based drug-eluting scaffolds have been studied comprehensively. METHODS AND MATERIAL: Nanoparticle-fibrin is prepared from thrombin and fibrinogen dissolved in NaOH and HCl. Then pomegranate powder is added to it. Nanoparticles/pom are provided by freeze drying and freeze milling. The 3-D scaffold of poly(lactide-co-glycolic acid) (PLGA) was prepared via salt-leaching solvent/casting leaching method and impregnated with nanofibrin-pom. Structural and chemical component of the scaffolds were evaluated by transmission and scanning electron microscopy and furrier transmission infrared spectroscopy, respectively. Moreover, the scaffolds were characterized from the degradation rate and drug releasing rate points of view of human Adipose Derive Stem Cells (hADSCs). Cytotoxicity effects of the scaffold were evaluated on hADSCs via MTT assay. RESULTS: The results showed that the size of nanoparticles was about 100 nm. The scaffold had a slow degradation rate and it caused a sustained release pattern of pom. MTT assay indicated that nanoparticles had no cytotoxicity and fibrin-pom nanoparticles increased compressive strength of PLGA/scaffolds dramatically and also caused a proper compressive modulus. CONCLUSIONS: By adding F/POM nanoparticle to PLGA and fabricating a three-dimensional nanocomposite scaffold (PLGA/F/POM nanoparticle), special physical and mechanical properties also suitable for drug release and cell behavior were achieved, which makes it suitable for cartilage tissue engineering applications (Tab. 1, Fig. 7, Ref. 53). Text in PDF www.elis.sk