Polycaprolactone-chitosan/multi-walled carbon nanotube: A highly strengthened electrospun nanocomposite scaffold for cartilage tissue engineering

Abstract

Articular cartilage is an avascular connective tissue with a slow healing rate. Tissue engineering scaffolds can provide appropriate condition to stimulate the natural healing mechanism of the damaged tissue. In this study, the electrospun nanocomposite scaffolds based on polycaprolactone (PCL)-chitosan/carboxyl-functionalized multi-walled carbon nanotubes (MWCNTs) were fabricated with different concentrations of MWCNTs including 0.5 and 1 wt. The samples were characterized in terms of morphology, porosity, physicochemical structure, hydrophilicity, tensile strength, bioactivity, biodegradation and cell response. The scaffold containing 0.5 wt MWCNTs presented the lowest fiber diameter (99 +/- 15 nm) and the highest tensile strength (33.81 +/- 6.76 MPa) (p <= 0.05), which were considerable for electrospun structures. The porosity percentage of the scaffolds were maintained above 80 which is appropriate for tissue engineering. As the MWCNTs increased, the water contact angle decreased due to the increase in the hydrophilic carboxyl functional groups related to the MWCNTs. MWCNTs increased the crystallinity of the scaffold, leading to a more bioactivity and stability proportional to healing rate of a natural cartilage. Chondrocytes were well cultured on the scaffold containing MWCNTs and presented more cell viability compared to the sample without MWCNTs. The PCL-chitosan/0.5wt.MWCNTs scaffold can be considered for supplemental studies in cartilage tissue engineering applications.