Study of the expression rates of RORA and COX5b genes amongst MS patients compared with healthy individuals as an emerging diagnostic biomarker

Abstract

Background: Multiple Sclerosis is a degenerative disease of the central nervous system accompanied with both environmental and genetic, backgrounds. The present survey was performed to assess the expression rates of COX5B and RORA genes in MS and healthy individuals. Methods: Sixty blood samples were taken from MS (n= 30) patients and healthy (n= 30) individuals. RNA was extracted from blood samples and used for cDNA synthesis. DNA samples were subjected to real-time PCR to assess the COX5B and RORA genes expression by specific primers. Results: MS patients had the higher history of smoking and alcohol. Statistically significant differences were obtained for the history of alcohol and smoking between MS and healthy population (P <0.05). There were no statistical differences between MS patients and healthy individuals for age, sex, BMI, and weight. COX5B gene expression was significantly decreased in MS patients compared to healthy individuals (P = 0.0433). RORA gene expression was increased in MS patients compared to the control group (P = 0.481). Conclusion: COX5B gene expression analysis can be used as an emerging biomarker for MS diagnosis in individuals. It seems that RORA gene expression should be studied more to find its specific role in MS pathogenicity.