Association between rs619586 (A/G) polymorphism in the gene encoding lncRNA-MALAT1 with type 2 diabetes susceptibility among the Isfahan population in Iran

Abstract

Background and aim: Type 2 diabetes mellitus (T2DM) is a global human disease that affects millions of people. Long non-coding RNAs (LncRNAs) are transcripts with more than two-hundred nucleotides that play essential roles in the management of mRNAs. In the present study, we examined whether the rs619586 (A/G) polymorphism in the gene encoding lncRNA-MALAT1 is associated with the susceptibility to T2DM among the Isfahan population, Iran. Methods: To this end, a case-control study was conducted on 200 healthy persons and 200 patients with T2DM. The genomic DNA was extracted from blood samples to strengthen the intended fragments containing rs619586 SNP. Using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP), the wild allele (A) and the mutant allele (G) were examined. Result and conclusion: Results indicated that the mutant allele (G) and mutant genotypes (AG/GG) were absent in T2DM patients. This absence suggests that the rs619586 (A/G) polymorphism in the gene encoding lncRNA-MALAT1 might not be associated with the susceptibility to T2DM among the Isfahan population. © 2021, Research Society for Study of Diabetes in India.