Dimethylaminoparthenolide (DMAPT) as an alternative approach for treatment of Familial Mediterranean Fever (FMF)

Abstract

Objective(s): Familial Mediterranean Fever (FMF) is a hereditary auto-inflammatory disorder that is caused by mutations in the Mediterranean fever (MEFV) gene and is associated with an increase in pro-inflammatory cytokines, such as interleukin-1 beta (IL-1 beta) and interleukin-18 (IL-18), leading to excess inflammation. Colchicine is a common drug widely used for treatment of FMF attacks, but about 5-15 of the patients show resistance to the regular colchicine treatment. In this study, we used dimethylamino-parthenolide (DMAPT), as a small molecule inhibitor of Nuclear factor-kappa B (NF-kappa B), NLR family Pyrin domain containing 3 (NLRP3), and cysteine-aspartic acid protease 1(Caspase-1) on FMF-derived peripheral blood mononuclear cells (PBMCs). Materials and Methods: The effects of DMAPT and colchicine on metabolic activity and apoptosis of FMF-derived PBMCs were evaluated by MTT and Annexin V/PI assays, respectively. Also, the expression levels of NF-kappa B, NLRP3, MEFV, CASP1, and IL-1 beta mRNA were investigated using a TaqMan real-time PCR, and the protein levels of IL-1 beta, IL-18, and IL-37 were assessed via an enzyme-linked immunosorbent assay (ELISA) in LPS/ ATP-stimulated PBMCs. Results: DMAPT decreased the expression levels of NFKB (0.38 +/- 0.096, P<0.0001), NLRP3 (0.39 +/- 0.12, P<0.001), MEFV (0.384 +/- 0.145, P<0.001), CASP1 (0.48 +/- 0.13, P=0.0023), and IL-1 beta (0.09 +/- 0.09, P<0.0001) and reduced the secretion levels of IL-1 beta (8.92 +/- 5.3 vs. 149.85 +/- 20.92, P<0.0001), IL-18 (135 +/- 32.1 vs. 192 +/- 22.18, P=0.01), and IL-37 (27.5 +/- 6.3 vs. 78.19 +/- 14.3, P<0.0001) as compared to untreated cells. Conclusion: Given the obtained results in comparison with previous research, the future clinical development of DMAPT could result in the expansion of new anti-inflammatory therapeutics for FMF disorder.