The frequency of candiduria in hospitalized patients at nephrology department, Labbafinejad hospital, Tehran, Iran

Abstract

Background: Candiduria is one of the most common infections in hospitalized patients, which is created by the administration of long-term antibiotic, disseminated candidiasis, urinary tract problems, and having long-term urine catheter. Prevalence of the candiduria in women is more than men. The aim of present study was to determine the frequency of the Candida species of candiduria in hospitalized patients in nephrology department, Labbafinejad hospital, Tehran, Iran. Methods: This study was carried out on 53 patients. The early identification of species was done using CHROMagar Candida medium. The genomic DNA was extracted using boiling method. Polymerase chain reaction (PCR) was used to amplify ITS1-5.8s-ITS2 region. The polymerase chain reaction products were then digested with MspI restriction enzyme. Polymerase chain reaction and restriction fragment length polymorphism (RFLP) products were revealed on 1.5 and 2 agarose gel electrophoresis, respectively, to identify the yeast. Findings: Among 53 patients, 14 were diagnosed with candiduria. In our study, the frequency of candiduria was 26.4. Separately, 5 patients (35.7) were men and 9 (64.3) were women. Candida glabrata (C. glabrata) had the highest frequency (42.8). Other identified Candida species were C. albicans (21.4), C. krusei (21.4), C. tropicalis (7.2), and C. parapsilosis (7.2). Conclusion: Increase in non-albicans species, due to resistance to antifungal drugs and increased use of immunosuppressive drugs, have resulted in many problems for candiduria treatment. Therefore, identification and appropriate treatment of these elements, especially in nephrology ward of hospitals, seems to be necessary. © Isfahan University of Medical Sciences(IUMS), 2017. All Rights Resreved.