Enterotoxigenicity, distribution of enterotoxigenic genes and antibiotic resistance pattern of Staphylococcus aureus isolated from traditional sweet

Abstract

Resistant and enterotoxigenic Staphylococcus aureus strains are considered to be one of the major causes of foodborne diseases due to the consumption of sweet. The present research was done to study the distribution of enterotoxin types, enterotoxigenic genes and antibiotic resistance pattern of S. aureus strains isolated from traditional sweet samples. Eight-hundred and fifteen sweet samples were cultured and S. aureus strains were identified. Antibiotic resistance, enterotoxigenicity and enterotoxigenic gene profile were studied using disk diffusion, Enzyme Link Immunosorbent Assay and PCR, respectively. One-hundred and seven out of 815 (13.12) sweet samples were positive for S. aureus. Prevalence of S. aureus in dried and semi-dried sweet samples were 15.08 and 11.13, respectively (P<0.05). Forty-six out of 107 S. aureus strains (42.99) were determined as enterotoxigenic. A (41.30) and C (17.39) were the most commonly detected enterotoxin types. Sea (20.56), sec (14.95) and seb (11.21) were the most commonly detected enterotoxigenic genes. There were no positive sample for the sej enterotoxin gene. S. aureus strains harbored the highest prevalence of resistance against penicillin (88.78), tetracycline (83.17), ceftaroline (75.70) and doxycycline (71.02). Simultaneous presence of enterotoxins and enterotoxigenic genes in multi-drug resistant S. aureus strains indicates important public health issue regarding the consumption of contaminated traditional sweet samples.