Evaluation of the mechanical characteristics of PLGA and PLGA/fibrin scaffolds in providing an appropriate environment for viability and growth of human adipose-derived stem cells

Abstract

Tissue engineered from mesenchymal stem cells (MSCs) requires a scaffold to keep the cells in the area defect and to act as a supporter for inducing tissue formation. We developed a three-dimensional (3-D) special poly-lactic-glycolic acid (PLGA) /fibrin (F) composite scaffold that provided structural supporter and stimulated repair. 3-D PLGA scaffold have been prepared via Solvent casting/salt leaching (SC/SL) techniques and the hybrid scaffold of PLGA/F was fabricated by F. Characterization techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and Scanning electron microscopy (SEM) were performed. Furthermore, mechanical properties of the PLGA and PLGA/F composite scaffolds were determined. 3-DPLGA and PLGA/F scaffolds seeded with cultured human adipose tissue-derived stem cells (hADSCs) and analyzed by MTT at 3 and 7 days. The results revealed that the scaffolds contain sufficient porosity with highly interconnected pore morphology. Increase the amount of fibrin enhanced compressive modulus and compressive strength of the PLGA scaffolds. The water absorption capacity difference for the PLGA scaffold with and without F. Our findings showed that PLGA/F scaffold in vitro, enhanced cellular viability was observed compared to PLGA scaffold in 3 and 7 days. © 2018 Society for Innovative Research. All rights reserved.