Differentiation of human adipose-derived stem cells toward neural cells using their conditioned medium

Abstract

Background: Human adipose-derived stem cells (hADSCs) are population of mesenchymal stem cells isolated from adipose tissue. Multipotent hADSCs can be differentiated into different cell lineages and they have ability to secrete variety of cytokines such as growth factors. These factors have neuroprotective effects and lead to survival and neural differentiation. The aim of this study was to investigate the effects of concentrated hADSCs conditioned medium (hADSCs-CM) on neural differentiation. Methods: The adipose tissue samples were obtained from young donors after signing the consent. The stem cells were isolated and cultured through several subcultures. In order to neural differentiation, the cells from 3rd to 5th passages were treated with hADSCs-CM for 21 days. The cells survival was assayed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and neural differentiation of cells was confirmed via immunocytochemistry. Findings: MTT assay indicated that there was significant difference (P < 0.050) in cell survival between treated and control groups at the days 7 and 21. The expression of neural marker β-tubulin III (β-TUB III) significantly increased in comparison to control group at the day 21. Conclusion: This study reveals that concentrated hADSCs-CM improves the survival and neural differentiation of hADSCs. Our results suggest that hADSCs-CM may pave the way for non-cellular treatment of neurodegenerative diseases. © 2017, Isfahan University of Medical Sciences(IUMS). All rights reserved.