An efficient deep eutectic magnetic nano gel for rapid ultrasound-assisted dispersive μ-solid phase extraction of residue of tetracyclines in food samples

Abstract

In the present study, a magnetic nano gel as the sorbent which is the combination of octatonic acid: cumarin as eutectic solvent and Fe3O4@SiO2 was introduced as the sorbent in ultrasound-assisted dispersive mu-solid phase extraction process coupled with high performance liquid chromatography with photo diode array detector for simultaneous separation and determination of tetracyclines residues in food samples. FT-IR, SEM, VSM were used for the characterization of the synthetized magnetic nano gel. Under obtained optimum conditions, the obtained linear ranges were 1.5- 500 (mu g L-1), 2.5-750 (mu g L-1), 2- 750 (mu g L-1), and 2.5- 500 (mu g L-1) for tetracycline, oxytetracycline, chlortetracycline, and doxycycline, respectively. Moreover, the below level of quantification (BLQ) (based on S/N = 3) of 0.47 mu g L-1, 0.11 mu g L-1, 0.85 mu g L-1, 0.66 mu g L-1, 0.81 mu g L-1 and the limit of quantification (based on S/N = 10) of 1.61, 2.74, 2.23 (mu g L-1), and 2.66 were achieved for tetracycline, oxytetracycline, chlortetracycline, and doxycycline, respectively. The intra-day and inter-day precision () of the procedure were less than 3.2 and 3.8, respectively. The recoveries over 95 confirmed high sufficiency of the proposed method for application in complex matrices such as honey, milk, and egg. GRAPHICS .