Inflammatory Responses of Women with Polycystic Ovary Syndrome in Vitro Differ from Healthy Women

Abstract

Polycystic ovary syndrome is a low-grade inflammatory state with increased serum levels of TNF-alpha. The present study has compared the inflammatory responses to breast cancer cell lines in women with PCOS with healthy women. Peripheral blood mononuclear cells (PBMCs) isolated from 50 women with PCOS and 50 healthy controls were cultured in the trans-well coculture system. These cells were stimulated with two distinct breast cancer cell lines. The proliferation of PBMCs, CD3+CD8+T cell percentages, and tumor necrosis factor-alpha (TNFa) concentration were evaluated after 48 and 72 hours of incubation. TNF-alpha concentration and the proliferation rate of PBMCs after 48 hours of incubation significantly increased in the PCOS group. However, after 72 hours, TNF-alpha secretion significantly decreased in the PCOS group. The ability of PBMCs to produce TNF-alpha decreased gradually in women with PCOS. When the effects of low-grade inflammation and endocrine conditions on the cells decrease, the inability of PBMCs to create an inflammatory response will be altered.