Renal cell carcinoma derived exosome as natural nanoparticles inhibits T cell proliferation and induces their apoptosis

Abstract

Objective(s): To evaluate the anti-tumor effect of the renal cell carcinoma ACHN cell-exosome on human T cell biological parameters. Methods: Human T cell was isolated from healthy donors. ACHN-cell derived exosome was procured and expression of the CD9 and CD63 was assessed using western blotting. The effects of the ACHN- exosome on T cell proliferation was measured using MTT assay within 24, 48, 72 and 96 hours of treatment with 500-2000 ng/ml exosome. Also, the apoptotic cell percentages in treated cells were assessed by Annexin/PI staining and flowcytometry upon exposure with 500 and 2000 ng/ml exosome within 96 hours of treatment. Results: According to results, exosome therapy inhibited T cell proliferation more evidently at higher concentration. Also, treatment with exosome caused an improvement in the apoptosis of the T cell within 96 hours of treatment. Conclusions: Concerning the results, ACHN- exosome has negative effect on T cell viability and proliferation, facilitating immune evasion. © 2023 The Author(s).