Identification of blaOXA-51-23-58, blaVIM, blaNDM, and blaIMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients

Abstract

ObjectiveThe increase of multidrug-resistant (MDR) strains of Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency of carbapenem resistance genes (oxacillinase and metallo-beta-lactamase) in A. baumannii.ResultsIn this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100 of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting blaOXA-51, blaOXA-23, blaOXA-58, blaNDM, blaIMP, and blaVIM genes PCR results revealed that among the selected isolates, 15 (60.0) harbored the blaOXA-23 gene, 23 (92.0) contained the blaOXA-51 gene, and 1 (4.0) isolate carried the blaNDM gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. blaOXA-51 and blaOXA-23 genes (oxacillinase genes) were dominant genes, but blaIMP and blaOXA-58 were not local carbapenem resistant genes in Isfahan.