Preparation and characterization of carboxymethyl cellulose/polyethylene glycol films containing bromelain/curcumin: In vitro evaluation of wound healing activity

Abstract

In this study, a wound-healing membrane was fabricated based on carboxymethyl cellulose (CMC) and polyethylene glycol (PEG) containing curcumin (Cur) and bromelain (Br). Citric acid (CA) was used as a cross-linking agent. This membrane showed an ideal degree of swelling, which was significantly dependent on the concentration and duration of cross-linked CA. The chemical characterization showed the CA cross-linker mechanism was more associated with chemical reactions with CMC carboxyl groups, and PEG hydroxyl groups played an important role in forming a hybrid polymer network. It greatly enhanced mechanical and adhesive properties, so the stress strength was improved from 29.4 to 38.52 Mpa. The hydrophilicity of the membrane surface according to the water contact angle assay showed the membrane surface is suitable for adhesion, growth, migration, and proliferation of skin cells. The drug delivery assay demonstrated that the Br and Cur were released during 48 h, but the Br followed the burst release in comparison with Cur. Antibacterial properties showed that CMC/PEG-Cur/Br have ideal antibacterial properties for preventing the growth of bacteria. In summary, the engineered CMC/PEG containing Cur/Br films with desired cell viability properties and antibacterial activity can potentially improve and accelerate skin regeneration for chronic wound healing.Highlights Hydrogel film was prepared based on carboxymethyl cellulose (CMC), polyethylene glycol (PEG), and citric acid as cross-linking agent. CMC/PEG film were induced controlling curcumin (Cur) and bromelain (Br) release for wound healing process. CMC/PEG film revealed a wide range of physiochemical, mechanical, adhesion, and biological properties behavior. Incorporation of Cur and Br promoted in vitro biocompatibility, L-929 cells attachments, and cell migration. The role of cross linking condition on drug release and biological responce of CMC/PEG wound dressing. image