Antimicrobial Effect of Curcumin Nanoparticles and Ferulago angulate Boiss Extract Against Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Wound Infections

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major causes of burn infections. Due to the increase of resistance in this bacterium, it is necessary to find new antimicrobial compounds. This study aimed to determine the antimicrobial effect of Curcumin nanoparticles and Ferulago angulate Boiss extract MRSA isolated from burns and other skin infections. Firstly, in addition to phenotypic microbial tests, the PCR technique for identification of the mecA gene was used to confirm MRSA strains. The MIC and checkerboard methods were used to investigate curcumin nanoparticles and Ferulago angulate Boiss extract effects against bacterial growth rate alone and in combination. The biofilm inhibitory effect was determined through the microtitre plate method. Also, the real-time PCR technique examined relative changes in eno gene expression expression. The MTT assay evaluated the cellular toxicity. The data obtained was analyzed through SPSS software and suitable statistical tests. Curcumin nanoparticles and F. angulate extract inhibited the growth of MRSA strains at a concentration of 312.5-625 mu g/mL, (MIC50 = 312.5). About 76.92 and 23.07 of clinical isolates showed indifferent and additive effects in combination with curcumin and F. angulate extract. The combination of curcumin and the mentioned extract decreased the eno gene expression compared to the 16S rRNA housekeeping gene. These two substances in combination and alone did not show any significant cytotoxic effects on HDF skin cell lines. In conclusion, curcumin nanoparticles and F.angulate extract showed some antibacterial effects against MRSA. Therefore, this compound should be considered with further tests against MRSA strains isolated from burn infections.