The Therapeutic Effect of the Combination of an Electron Beam of 6 Megaelectron Volts and Cisplatin on the B16F10 Mouse Melanoma Cell Line

Abstract

Background: Melanoma cancer is the most dangerous and deadly skin cancer, for which preventive treatments such as surgery, radiation therapy, and chemotherapy have been suggested. In this study, we tried to investigate the effect of the simultaneous use of electron beam and cisplatin drug. Methods: Cells in DMEM-F12 culture medium containing 10 fetal bovine serum (FBS) and streptomycin and penicillin antibiotics in an incubator at 37°C and 5 carbon dioxide in the amount of 2×104 They were cultured to perform the MTT test. The cytotoxic effect of Cisplatin with concentrations of 0.6, 1.5, 5, 10, and 20 μg/ml and electron beam of 1, 2, and 3 Gy was measured separately by MTT test and, then the effect of the combined treatment on cells was measured using all drug concentrations and radiation doses. The statistical tests for drugs, radiation, and their combination were fully performed. Findings: Based on statistical tests, increasing the concentration of the drug alone or in combination with an electron beam increased the level of toxicity, and this significant increase was reported. Also, increasing the dose of the drug separately and in combination with the drug significantly increased the toxicity gave. Conclusion: The combination of two treatment methods can prevent the use of high concentrations of drugs or high doses of electron beams and significantly reduce the application limitations and side effects of drugs and radiation. © 2024 Isfahan University of Medical Sciences(IUMS). All rights reserved.