Galectin-9 induces IL-1β production as a key inflammatory cytokine in the acute myeloid leukemia cell line (U937)

Abstract

Background and purpose:T-cell immunoglobulin and mucin-domain containing protein-3 (TIM-3)/ galectin-9 (Gal-9)/ autocrine loop in myeloid leukemia stem cells provokes inflammation through the NF-kappa B signaling pathway, which is influential in the expression of inflammatory factors. Interleukin 1 beta (IL-1 beta) is a vital inflammatory cytokine that plays an important role in the proliferation and therapy resistance of acute myeloid leukemia (AML) cells. This study aimed to assess the effect of Gal-9 on IL-1 beta in the human leukemic U937 cell line.Experimental approach:The U937 cells were cultured in different concentrations of Gal-9. Cell counting kit-8 was used to assess the effect of Gal-9 on human leukemic U937 cell proliferation. Also, its impact on the expression of TIM-3, Gal-9, IL-1 beta, IL-1 beta R, IL-1 beta RAP, and NLRP3 genes and IL-1 beta protein was studied by RT-PCR and ELISA, respectively. Moreover, the effect of Gal-9 on the NF-kappa B signaling pathway was evaluated by western blotting.Findings/Results:U937 cells were expanded in the presence of Gal-9 in a concentration-dependent manner. Following treatment of U937 cells with Gal-9, the gene expression of Gal-9, IL-1B, IL-1BR, and IL-1BRAP were significantly upregulated compared to the control group. The IL-1 beta concentration increased following Gal-9 treatment in a concentration-dependent manner, while following time-pass its level significantly decreased. Furthermore, Gal-9 slightly increased NF-kappa B phosphorylation.Conclusion and implications:Gal-9 increased IL-1 beta level as a critical inflammatory cytokine in the proliferation and resistance of AML cells to therapy. According to this finding, targeting and blocking the TIM-3/Gal-9 autocrine loop can suppress IL-1 beta production and facilitate AML treatment.