Introduction of a novel graphene oxide-based nickel nanoflowers for ultrasound-assisted dispersive solid phase extraction of natamycin from dairy products

Abstract

This study presented facile ultrasound assisted dispersive solid-phase extraction coupled with high performance liquid chromatography-diode array detector for preconcentration and determination of trace levels of natamycin in dairy products. Highly efficient and selective graphene oxide-based nickel nanoflowers were synthesized and characterized with FE-SEM-EDX, FT-IR, TGA, and XRD techniques. The leading factors including pH, ionic strength, sample volume, the amount of sorbent, and desorption conditions were studied using matrix matched method. Good linearity of 1-750 mu g kg-1 for milk, 1-500 mu g kg-1 for yogurt and cheese were acquired. While 0.30, 0.27, and 0.30 mu g kg-1 as LODs values and 1.00, 0.93, and 0.97 mu g kg-1 as LOQs values were obtained for milk, cheese, and yogurt, respectively. The intra-day precision (n = 7) and the inter-day precision (5 days, n = 3) were achieved <= 4.1 and <= 3.0, respectively, confirming the method's repeatability and reproducibility. High recoveries of 94.0-98.4 and also the satisfactory obtained results indicated high capability of the proposed method for separation and preconcentration of natamycin be applied in dairy products as complex matrixes.