Investigation of Fisetin Effects on Serum Immunoglobulin G Levels and Maintenance of Locomotor and Balance Posture in Mice, a Toxic Model of Myelin Tissue Destruction

Abstract

Background: Common molecular mechanisms are involved in the pathogenesis of neurodegenerative diseases. Compounds that can simultaneously target multiple molecular mechanisms are typically used to treat these diseases. In the present study, the effects of fisetin on serum immunoglobulin G levels and the maintenance of motor coordination and balance in mice were investigated. Methods: In this study, 20 C57BL/6 mice were randomly divided into 5 groups including control, sham, cuprizone, fisetin, and cuprizone/fisetin. Motor coordination and balance impairment was induced using cuprizone. In the groups receiving fisetin, this compound was injected intraperitoneally at a dose of 20 mg/kg daily. Serum immunoglobulin G levels were evaluated using the ELISA method, and motor coordination and balance were assessed using the hanging wire test. Finally, data were analyzed using One Way ANOVA. Findings: The mean expression level of the IgG factor in the fisetin-treated groups showed a significant decrease compared to the cuprizone group (P ≤ 0.01). Also, the mean score in the hanging wire test was significantly increased in the fisetin-treated groups compared to the cuprizone group (P ≤ 0.05). Conclusion: Exposure to toxic compounds such as cuprizone can lead to increased IgG expression and cause balance motor problems. Therefore, the use of flavonoids such as fisetin, whose immunomodulatory, antioxidant, and neuroprotective effects have been proven, can play an important role in reducing these complications. © 2025, Isfahan University of Medical Sciences(IUMS). All rights reserved.