A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System

Abstract

BACKGROUND: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. METHODS: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells. RESULTS: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis. CONCLUSION: The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.