The study of TAAAA repeat polymorphism in p53 gene and its association with prostate cancer

Abstract

Background: Prostate cancer has a high degree of heterogeneity in pathology and clinical appearance. In response to stress, P53 plays an important role in preventing cancer development. To our knowledge, this is the first report about polymorphic TAAAA repeat of the P53 gene and its relation to prostate cancer risk. The purpose of this study was to investigate polymorphism of TAAAA in the first intron of p53 gene among prostate cancer patients and healthy individuals and its relation to risk of prostate cancer. Methods: A total of 306 peripheral blood samples consisting of 156 from patients with prostate cancer and 150 from healthy control individuals were included in the study. DNA was extracted from blood using salting-out method. TAAAA repeat sequences were amplified by PCR technique and the length of products was determined by polyacrylamide gel and direct sequencing. Findings: Based on our results, 5 alleles and 12 different genotypes for P53 TAAAA repeat polymorphism were observed. The most common allele in both patients and controls was the allele 8/8. Men who carry 9/9 or 10/10 genotypes of p53 gene are at significantly higher risk of prostate cancer. The allelic length of p53 polymorphisms had no significant effect on age of onset, inheritance as well as metastasis. Conclusion: Our study showed a strong association between the TAAAA repeat polymorphism in P53 gene and risk of prostate cancer. � 2016, Isfahan University of Medical Sciences(IUMS). All rights reserved.