Molecular Studies on Cystic Echinococcosis of Camel (Camelus dromedarius) and Report of Echinococcus ortleppi in Iran

Abstract

Background: Cystic echinococcosis (CE) is one of the most important zoonotic diseases; caused by different genotypes of Echinococcus spp. Camels have an important role in transmission cycle of E. granulosus especially, in desert areas. This study aimed to investigate molecular characterization of hydatid cysts isolates from onehumped camel (Camelus dromedarius) and to show its molecular and phylogenic status in this important CE host in the central part of Iran. Methods: Twenty hydatid cyst samples (14 fertile and 6 calcified) were collected from 56 slaughtered camels in Central part of Iran. Extraction of DNA from 14 fertile samples was achieved followed by molecular studies on two mitochondrial genes (nad1 and cox1). Results: Blast and phylogenetic analysis on sequenced genes showed the presence of G1 (28.6), G3 (28.6) and G6 (35.7) genotypes in the samples. However, one sample was detected as E. ortleppi (G5) with 99 homology with G5 isolated from camel in Egypt (AB921055) and Sudan (JX912709). Conclusion: Presence of E. ortleppi, originally the cattle genotype, is reported for the first time in Iran. Due to the potential of infecting human by E. ortleppi; more attention should be paid to this zoonotic genotype in this region.