Assessment of hydroalcoholic extract of seeds and leaves of Moringa peregrina on ileum spasm

Abstract

Seeds of Moringa peregrina (Forssk.) Fiori. (Moringaceae) is widely used in south east of Iran for gastrointestinal disorders. However, so far there is no pharmacological evidence for antispasmodic activity of this plant extract. Therefore, the aim of this research was to investigate antispasmodic activity of M. peregrina on rat isolated ileum contraction. Hydroalcoholic extract was obtained by percolation method from seeds and leaves of M. peregrina collected from Baluchestan province of Iran. A portion of isolated rat ileum was suspended under 1 g tension in Tyrodes solution at 37 degrees C and gassed with O 2 . Effects of seeds and leaves extracts of M. peregrina were studied on ileum contractions induced by KCl (80 mM), acetylcholine (ACh, 250 mu M) and electrical field stimulation (EFS). The seed extract of M. peregrina concentration dependently inhibited the response to KCl (IC 50 =87 18 g/ml), ACh (IC 50 =118 18 g/ml) and EFS (IC 50 =230 51 g/ml). The extract of M. peregrina leaves also had inhibitory effect of ileum contraction induced by KCl (IC 50 =439 108 g/ml), ACh (IC 50 =365 61 g/ml) or EFS (IC 50 =314 92 g/ml). From these experiments it was concluded that M. peregrina extract mainly had an inhibitory effect on ileum contractions but the seed extract was more potent than the leave extract in inhibiting KCl and ACh contractile responses.