Evaluating T-cell immunoglobulin mucin-3 (Tim-3) receptor in the growth inhibition of acute lymphoblastic leukemia (all) cell lines

Abstract

Background: T-cell immunoglobulin-mucin (TIM) is a cell-surface and transmembrane glycoprotein. TIM-3 plays a pivotal role in proliferation, invasion and metastasis of tumor cells. The present study was designed to evaluate the expressions of the TIM-3 and the role of the galectin-9, as TIM-3 ligand, in the regulation of cell proliferation in human acute lymphoblastic leukemia (ALL) cell lines. Methods: The expression level of TIM-3 was examined in the Jurkat and KE-37 cell lines using realtime polymerase chain reaction method. MTS 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium viability test was used to study the cell proliferation effect of galectin-9. Findings: TIM-3 mRNAs were detected in the both Jurkat and KE-37 cell lines. The expression of TIM-3 in Jurkat cell line was higher than KE-37 cell line (P < 0.001). The MTS assay revealed that galectin-9 reduced cells proliferation in a dose-dependent manner (> 1 nM) in the both cell lines (P < 0.050). Conclusion: The present investigation introduced a possible mechanism for the control of acute lymphoblastic leukemia cell proliferation through TIM-3 and demonstrated that galectin-9 can inhibit the proliferation of Jurkat and KE-37 cell lines. © 2015, Isfahan University of Medical Sciences(IUMS). All rights reserved.