miR-370 expression analysis in breast cancer

Abstract

Background: MicroRNAs (MiRNAs) are 21-24 nucleotides which have different levels of expression between tumors and normal tissues. In this study, we analyzed the expression level of miR-370 in different stages and grades of human breast cancer tissues compared with adjacent normal tissues. Methods: In this case-control study, the expression of miR-370 on 22 tissue samples (tumor and normal) from patients with breast cancer was investigated via quantitative real-time polymerase chain reaction method. Findings: miR-370 expression showed a significant increase in tumor tissues compared to normal tissues (P = 0.001) as well as in cancer tissues at stage III (P = 0.004) and grade 3 (P = 0.011) than in other stages. Conclusion: According to evaluation of miR-370 expression in different stages and grades and its relation to more advanced tumor stages/grades, it can possibility act as oncomiRNA in breast cancer. It may be suggested as a prognostic biomarker or therapeutic target in breast cancer, too. © 2015, Isfahan University of Medical Sciences(IUMS). All rights reserved.