Cell viability and neural markers expression of human adipose-derived stem cells after neural induction in alginate tridimensional cell culture

Abstract

Background: Different studies have been done to obtain sufficient number of neuronal cells for treatment of central nervous system injuries. Adipose-derived stem cells have higher proliferation rate and more resistant to apoptosis. These cells can be induced to differentiate along several multilineage cells such as neuron-like cells. Alginate hydrogel is a polysaccharide polymer that has proper properties including biocompatibility with no immunogenicity. In this study, the effect of alginate hydrogel on the cell viability, proliferation rate and neurogenic differentiation of human adipose derived stem cells was evaluated at 14 days after induction. Methods: Adipose-derived stem cells isolated from human, were cultured in neural induction media and seeded into alginate hydrogel. The cell viability and differentiation efficiency of encapsulated cells were evaluated via doubling time and real time quantitative reverse transcription polymerase chain reaction (real time RT-PCR) analysis, respectively. The collected data was analyzed using oneway ANOVA test. Findings: The rate of proliferation of encapsulated cells significantly decreased as compared with control group (P < 0.001). The expression of nestin, glial fibrillary acidic protein (GFAP) and microtubule-associated protein 2 (MAP2) markers significantly increased as compared with control group (P < 0.001). Conclusion: Altogether proliferation rate of encapsulated cells in alginate hydrogel significantly decreased as compared with control group, but alginate hydrogel can promote neural differentiation of adipose-derived stem cells. © 2015, Isfahan University of Medical Sciences(IUMS). All rights reserved.