Investigation of the association between (CCTTT)n polymorphism in NOS2 gene and serum IgE level in patients with vernal keratoconjunctivitis in Chaharmahal va Bakhtiari Province

Abstract

Introduction: Vernal keratoconjunctivitis (VKC) disease is an immune response of the body caused by environmental antigens leading to the inflammation of the conjunctive tissue. Many factors have been regarded as potential contributing factors in VKC development. NOS2 gene is one of the genetics factors that can be supposed as a potential contributor in the disorder development. In this research, the association between (CCTTT)n polymorphism in NOS2 gene promoter was investigated to find the presumable emolument. In the Iranian populations have not any reported the association of polymorphism CCTTT (n) with the promoter of the NOS2 gene. Methods and materials: The study included 200 individuals (100 patients with VKC and 98 controls). After DNA extraction, PCR was performed following a standard phenol chloroform protocol, and then Genotypes were identified using polyacrylamide gel electrophoresis (PAGE). Serum IgE levels were estimated using Elisa Kit. Interpretation of findings was accomplished using SPSS software after analyzing the quality and quantity criteria associated with the signs and symptoms of the disease. Results: (CCTTT)n polymorphism allele analysis in our study showed 10 alleles in patient group and 11 alleles in control group. The difference of allele (CCTTT) frequency between the patient and control groups was significant (P-value < 0.001). Serum IgE levels in both groups did not show statistically significant differences (P-value=23). Conclusion: In this study, we verified the association of (CCTTT) 12 and VKC disease. Therefore, the concerned nucleotide change can be employed as an informative tool in determining the disease prognosis and detection of carriers, identification tests and elucidating the mechanism by which VKC develops.