A novel nano-composite scaffold for cartilage tissue engineering

Abstract

In this study, hybrid Poly (lactic-co-glycolic acid) (PLGA)/Hyaluronic Acid (Ha)/Fibrin/45S Bioactive Glass (45SBG) nanocomposite scaffolds seeded with human Adipose-Derived Mesenchymal Stein Cells (hADMSCs) were investigated as a construct for osteoarthritis (OA), Articular Cartilage (AC), and subchondral bone defects therapies. The bioactivity and biodegradation of the nanocomposite scaffolds were assessed in Simulated Body Fluid (SBF) and Phosphate Buffer Saline (PBS) solution, respectively. Furthermore, MITT analysis was performed in order to determine attachment and viability of hADMSCs. Ultimately, results indicate the increase of bioactivity in nanocomposite scaffolds, as compared to the pure PLGA scaffold. In addition, biodegradation assay exhibits that the addition of Ha, fibrin, and 45SBG nanoparticles could modify the degradation rate of PLGA. The nanocomposite scaffolds did not exhibit any cytotoxicity, and the hADMSCs were attached to the scaffolds , which proliferate properly. According to our investigation, it was concluded that using natural and synthetic polymers along with BG nanoparticles may provide a suitable construct and could show a beneficial role in AC tissue engineering and OA therapy. (C) 2018 Sharif University of Technology. All rights reserved.