Chondrogenic differentiation of human scalp adipose-derived stem cells in Polycaprolactone scaffold and using Freeze Thaw Freeze method

(2018) Chondrogenic differentiation of human scalp adipose-derived stem cells in Polycaprolactone scaffold and using Freeze Thaw Freeze method. Journal of Cellular Physiology. pp. 6705-6713. ISSN 0021-9541

Full text not available from this repository.

Abstract

Human adipose tissue has been identified as a viable alternative source for mesenchymal stem cells. SADSCs were isolated from human scalp biopsy and then were characterized by Flow cytometry. SADSC(S) expressed CD90, CD44, and CD105 but did not express CD45 surface marker. Growth factors were used for chondrogenesis induction. Histology and immunohistology methods and gene expression by real-time PCR 14 days after induced cells have shown the feature of chondrocytes in their morphology and extracellular matrix in both inducing patterns of combination and cycling induction. Moreover, the expression of gene markers of chondrogenesis for example collagen type II aggrecan and SOX9 has shown by real-time PCR assay. Then, SADSCs were seeded alone on polycaprolatone (PCL) and with Freeze thaw Freeze (PCL+FTF) scaffolds and SADSCs differentiated toward the chondrogenic lineage and chondrogenesis induction were evaluated using scanning electron microcopy (SEM) and MTT assay. Our results showed that SADSCs were also similar to the other adipose-derived stem cells. Using TGF-beta3 and BMP-6 were effective for chondrogenesis induction. Therefore using of TGF-beta3 and BMP-6 growth factors may be the important key for in vitro chondrogenesis induction. The bio-composite of PCL+FTF nanofibrous scaffolds enhance the chondroblast differentiation and proliferation compared to PCL scaffolds .Therefore, our model will make it possible to study the mechanism of transition from chondroblast to chondrocyte. Our model will make it possible to study the mechanism of transition from chondroblast to chondrocyte.

Item Type: Article
Keywords: chondrogenic differentiation freeze thaw freeze polycaprolactone scalp adipose-derived stem cells platelet-rich plasma human articular chondrocytes growth-factor bone-marrow in-vitro tissue expression induction knee proliferation
Divisions: Faculty of Medicine > Department of Basic Science > Department of Molecular Medicine and Genetics
School of Advanced Technologies in Medicine > Department of Biomaterials, Nanotechnology and Tissue Engineering
Page Range: pp. 6705-6713
Journal or Publication Title: Journal of Cellular Physiology
Journal Index: ISI
Volume: 233
Number: 10
Identification Number: https://doi.org/10.1002/jcp.26477
ISSN: 0021-9541
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/6998

Actions (login required)

View Item View Item