Risk factor effect of rs1044165 and rs3745453 as neighboring variants of miR-223, miR-24, miR-23a and miR-27a on the onset of MS disease in Isfahan/Iran

Abstract

Multiple sclerosis (MS) is a multifactorial, chronic and inflammatory disease due to the myelin sheath damage of the central nervous system (CNS). Genome-wide association studies reveal that progression of MS is associated with single nucleotide polymorphisms (SNP). To elucidate the frequency of two SNPs (rs3745453 and rs1044165) with the incidence of MS, a population composed of 310 MS patients and 280 healthy volunteers were selected in Isfahan as a high prevalent location in the heart of Iran. After genomic extraction of blood samples, Allele specific primer PCR (ASP-PCR) was implemented for genotyping of the samples. Data indicated that frequency of carriage of CC genotype in rs3745453 in MS patients compared to healthy individuals and frequency of carriage of rs1044165 TT genotype increased in female MS patients compared to the controls. Logistic regression analyses indicated a risk factor role of rs1044165 and rs3745453 in the pathogenesis of MS.