The effect of lipopolysaccharide on the expression level of immunomodulatory and immunostimulatory factors of human amniotic epithelial cells

(2018) The effect of lipopolysaccharide on the expression level of immunomodulatory and immunostimulatory factors of human amniotic epithelial cells. BMC Res Notes. p. 343. ISSN 1756-0500 (Electronic) 1756-0500 (Linking)

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Abstract

OBJECTIVE: Human amniotic epithelial cells (hAECs) are a novel source of stem cells and have immunomodulatory effects on both the innate and adoptive immune system. hAECs can differentiate into multiple cell lineages that make them a suitable cell source for regenerative medicine. These cells express multiple toll-like receptors (TLRs) and respond to various TLR ligands. This study aimed to evaluate the effect of lipopolysaccharide (LPS), a TLR4 ligand, on the level of immunomodulatory and immunostimulatory factors of hAECs. RESULTS: Our results indicated that LPS had the ability to up-regulate the expression of prostaglandin E2 synthase and transforming growth factor-beta1 in hAECs. However, there was no change in the level of interleukin-1beta, interleukin-6 and interleukin-10 in hAECs when were stimulated with LPS. In addition, we observed tumor necrosis factor-alpha was only expressed at very low level in some of hAECs samples which its expression was independent of the effects of LPS.

Item Type: Article
Keywords: Adult Amnion/*cytology Cytokines/*drug effects *Epithelial Cells/drug effects/immunology/metabolism Female *Fetal Stem Cells/drug effects/immunology/metabolism Humans Immunologic Factors/*pharmacology Lipopolysaccharides/*pharmacology Pregnancy Prostaglandin-E Synthases/*drug effects Human amniotic epithelial cells Immunomodulatory effects Lipopolysaccharide Regenerative medicine Toll-like receptors
Divisions: Faculty of Medicine > Department of Basic Science > Immunology Department
Page Range: p. 343
Journal or Publication Title: BMC Res Notes
Journal Index: Pubmed
Volume: 11
Number: 1
Identification Number: https://doi.org/10.1186/s13104-018-3411-9
ISSN: 1756-0500 (Electronic) 1756-0500 (Linking)
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/8029

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