Evaluation of the therapeutic effect of 6-MV X-ray radiation on HeLa cells, in the presence of nanoparticles

Abstract

Background: Today, the use of a combination methods to increase the effectiveness of treatment is considered very much. One of these methods is the use of X-rays in the presence of nanoparticles. In this study, the effect of 6-MV X-ray radiation on the viability of HeLa cells, in the presence of gold nanoparticles, was investigated. Methods: HeLa cells were cultured and divided in four groups including control, gold nanoparticle, X-ray, and X-ray in presence of gold nanoparticle. In the gold nanoparticle group, the cells were incubated with 0.2, 1, and 5 μg/ml for 24 hours. In the X-ray group, the cells were exposed to X-ray with energy of 6 MV, and doses of 0.5, 1, and 2 Gy. In the group of X-ray in presence of nanoparticles, the cells were first incubated with nanoparticles for 24 hours, and then exposed to X-ray radiation under the same conditions. The viability of cells was measured using MTT assay at 24 and 48 hours after the intervention. Findings: In the group of X-ray in the presence of nanoparticles, the viability of the HeLa cells reduced up to 50 at both 24 and 48 hours, in different concentrations. With the increase in concentration in a certain dose, cell survival could change up to 15, but dose change was only significant in 2 Gy compared to other doses. Conclusion: The viability of the HeLa cells during X-ray radiation, in the presence of gold nanoparticles, has a significant reduction. It can be concluded that simultaneous use of gold nanoparticles and X-ray increases cell death. © 2018, Isfahan University of Medical Sciences(IUMS). All rights reserved.