CDH1 gene mutations and its association with diffuse gastric cancer

Abstract

Background: Gastric cancer (GC) is one of the common cancers in world and second cause of cancer death. CDH1gene mutations are the important cause of diffuse gastric cancer. E-cadherin protein is coded by CDH1 gene. In present study, we assess the nucleotide changes in CDH1 gene among diffuse gastric cancer patients. Materials and methods: We assessed 45 patients of gastric cancer (17 hereditary and 28 sporadic) that recognized according to the histopathological criteria and family history. DNA extracted from formalin fixed paraffin-embedded tissues and blood samples. The entirecoding region of CDH1 gene was amplified by polymerase chain reaction. Results: Totally, 45 patients including 7 males and 10 females in hereditary diffuse gastric cancer (DGC) and 8 female and 20 male in sporadic DGC were assessed. In hereditary DGC cases in 17.6 patients tumors were recognized in early TNM stage (I, II) versus late stages (III, IV) in 82.4. In sporadic DGC cases 21.4 were stage (I, II) and 67.9 stages (III, IV) and 10.7 unknown. Overall, 16 variants (7 exonic and 9 intronic) was detected in HDGC patients. Contrary, sixteen variants (3 exonic and 13 intronic) were found in CDH1 gene. Conclusion: Totally, 64.7 of hereditary and 14.2 of sporadic samples was exhibited CDH1exonic variants. Our results reveal that should be considered even one cases of diffuse gastric cancer with positive family history that prominence important regarding genetic counselling.