Post-transcriptional gene regulation with locked nucleic acid anti-miR-92a-3p enhances tumor protein p63 expression in human colorectal cancer

Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNAs that negatively regulate post-transcriptional gene expression by binding to the 3 '-untranslated regions (3 ' UTR) of messenger RNA (mRNA). In recent years have been identified that aberrant expression of miRNAs correlated with cancer progression. Colorectal cancer (CRC) is oneof the most cancer-related deaths malignancies in human thatmiRNAs can dramatically effect on its progression. The aim of this studywas to evaluatethe inhibitory effect of miR-92a-3p on SW 48 cells (human CRC cell line) and its effects on theexpression oftumor protein P63. Studies demonstrate that miR-92a-3p is overexpressed in colorectal cancer. Here with using Locked Nucleic Acid (LNA) anti-miR-92a-3p we suppressed the expression of this miRNA. We quantified the amount of miR-92a-3p in three-timeintervals and determine that LNA-anti-miR significantly inhibits this miRNA, we have also determined that inhibition of miR-92a-3p enhanced the amount oftumor protein p63 expression a member of p53 homologue that involves in cell cycle arrest, differentiation and apoptosis in cells. This it might be a promising finding for understanding CRCmechanisms, etiology and planning strategy for treatment of this cancer.