Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells

(2018) Antiproliferative effect of upregulation of hsa-let-7c-5p in human acute erythroleukemia cells. Cytotechnology. pp. 1509-1518. ISSN 0920-9069

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Abstract

New achievements in the field of cancer treatment are results of recent advances in molecular medicine and gene therapy. Usage of microRNAs (miRNAs) which are small noncoding RNAs is one of the molecular research lines for the diagnosis and treatment of cancers. miRNAs have an important role in post-transcriptional regulation of the gene expression and are involved in cellular activities such as growth, differentiation, cell death and cancer development. One of the miRNAs that showed downregulation in human acute erythroleukemia is hsa-let-7c-5p. Down-regulation of hsa-let-7c-5p has been reported in in vitro studies of different cancers. In the present study, upregulation of hsa-let-7c-5p is performed in human acute erythroleukemia cell line (KG-1) using miRNA mimic. qRT-PCR, MTT assay, Annexin-V, and propidium iodide staining at different time points after miRNA mimic transfection were accomplished to assess the expression level of hsa-let-7c-5p, cell viability, apoptosis and late apoptosis. In addition, the expression level of PBX2 oncogene, a validated target gene of hsa-let-7c-5p, is evaluated by RT-qPCR to show the effectivity of this approach on erythroleukemia cancer cells. Our results can be used in translational medicine for future investigation in acute erythroleukemia and to approach treatment based on miRNA mimic therapy.

Item Type: Article
Keywords: erythroleukemia hsa-let-7c-5p mirna mimic proliferation apoptosis acute myeloid-leukemia micrornas cancer adults aml proliferation suppressor diagnosis differentiation recommendations
Divisions: Faculty of Medicine > Department of Basic Science > Department of Molecular Medicine and Genetics
Page Range: pp. 1509-1518
Journal or Publication Title: Cytotechnology
Journal Index: ISI
Volume: 70
Number: 6
Identification Number: https://doi.org/10.1007/s10616-018-0241-5
ISSN: 0920-9069
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/9282

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