Effect of Moisture on the Root Treated Canals' Microleakage Using Two Types of Resin Based Sealers

Abstract

Aim: To estimate the stature of an individual using both clinical and radiological methods and to evaluate the most accurate method in estimating stature of an individual. Materials & Method: 36 human canines were extracted and decoronated to achieve a 15 ram root length and were assigned to 4 groups randomly. All teeth were instrumented using the step-back technique and 2.5 sodium hypochlorite was used as an irrigation solution in this protocol. The groups were organized to have 18 teeth for each sealer, half of which belonged to the group of moistened teeth and the other half to the group of the dried teeth. In this study, AH-plus and AH26 (Dentsply/Middle East and Africa) were used as two common resin sealers. To evaluate the microleakage, the bacterial evaluation system was used. The roots were placed in 36 end-removed micropipettes, sealed and strelized with ethylene oxide gas. One ml of the solution containing E. faecalis (ATCC 29212) (the most prevalent and strongest bacteria in endodontics with the minimal size) was injected from the upper part of the system every three days for 90 days and microleakage was evaluated daily by observing turbidity in the BHI solution. Results: The results showed microleakage in all the samples over 40 days and the survival test analysis demonstrated different times of microleakage in the groups. The longest mean time of microleakage was related to group 2 (AH26 dry) and the shortest mean time was related to group 3 (AH-plus wet). Conclusion: The results of this experimental study showed that among the four groups of the study, the highest resistance to microleakage belonged to group 2 (AH26 dry) and the least resistance belonged to group 3 (AH-plus wet). This difference in resistance was not significant between other grou.