Evaluation of sustained ciprofloxacin release of biodegradable electrospun gelatin/poly(glycerol sebacate) mat membranes for wound dressing applications

Abstract

In this research, gelatin and poly(glycerol sebacate) (PGS), which have demonstrated significantly developed wound healing process, were used to fabricate biodegradable and bioabsorbable membranes with controlled release of ciprofloxacin (CIP) as a powerful antibiotic. This was achieved by taking advantage of electrospinning technique to accelerate the wound healing as an extracellular matrix-liked composite membrane. We attempted to carefully investigate the impact of variable ratio of sebacic acid and glycerol as components of PGS and identified CIP-loaded cross-linked membrane (CIP-M-11) as an optimum membrane, although a significant change in structural properties of samples was not observed. The physicochemical properties of drug-loaded electrospun wound cover were evaluated by several characterization tests. Scanning electron microscopy and Fourier transform infrared spectroscopy results confirmed that CIP-embedded gelatin/PGS membrane was successfully synthesized and cross-linked properly. The CIP-M-11 sample showed a constant and uniform degradation rate with the capacity of durable drug release over the time period. The excellent antibacterial activity of CIP decreased the population of both Escherichia coli and Staphylococcus aureus bacteria and prevented bacterial infections. The cytocompatibility of membranes was completely proven by culturing L929 fibroblast cells on the prepared membranes. Finally, obtained results exhibit that the membranes should be considered as the promising wound dressing.