Galectin-9 induces apoptosis in OVCAR-3 ovarian cancer cell through mitochondrial pathway

(2018) Galectin-9 induces apoptosis in OVCAR-3 ovarian cancer cell through mitochondrial pathway. Research in Pharmaceutical Sciences. pp. 557-565. ISSN 1735-5362

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Abstract

Galectin-9 (Gal-9), a member of animal lectins' family, is implicated in the induction of apoptosis in various cancer cells. Here, we evaluated the anti-tumor effect of Gal-9 in OVCAR-3 ovarian cancer cells. The effect of the Gal-9 on cell viability was evaluated using MTT assays. Apoptosis was assessed using Annexin-V staining. The assessment of mitochondrial membrane potential (Delta Psi m) was performed using a JC-1 probe. The activity of caspase-3 and caspase-6 were evaluated with colorimetric assay. The production of reactive oxygen species (ROS) was applied by fluorescent probe. The expression levels of Bax and Bcl-2 were assessed using western blotting. The result showed that Gal-9 inhibits cell viability. Flow cytometry analysis showed that Gal-9 induces apoptosis in ovarian cancer cells. Moreover, Gal-9 decreased Delta Psi m and increased the generation of ROS and caspase-3 and caspase-6 activities in ovarian cancer cells. Moreover, Gal-9 induced expression of Bax as well as inhibited expression of Bcl-2. In conclusion, our results indicated that Gal-9 induced apoptosis in ovarian cancer cells through mitochondrial pathway.

Item Type: Article
Keywords: apoptosis gal-9 ovarian cancer ovcar-3 reactive oxygen species cycle arrest stem-cells receptor lines suppresses induction caov-4
Divisions: Faculty of Pharmacy and Pharmaceutical Sciences > Department of Clinical Biochemistry
Other
Page Range: pp. 557-565
Journal or Publication Title: Research in Pharmaceutical Sciences
Journal Index: ISI
Volume: 13
Number: 6
Identification Number: https://doi.org/10.4103/1735-5362.245967
ISSN: 1735-5362
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/9500

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