The Effect of Genistein, L-carnitine and their combination on gene expression of hepatocyte PPAR-alpha and CPT-1 in experimental nephrotic syndrome

Abstract

Objective: One of the disorders that occur in nephrotic syndrome would be hyperlipidemia. L-carnitine and genistein have the capability to control this syndrome by changing in lipid metabolism. In the present study, we delved into the effects of Genistein and L-carnitine on the PPAR-alpha and CPT-1 gene expressions in experimental nephrotic syndrome. Methods: In controlled experimental study, 50 male Sprague- Dawley rats were randomly divided into five groups consisting of 10 animals each with similar mean body weights (300 +/- 50 g): NC (normal-control), PC (patient-control), LC (L-carnitine), G (genistein), and LCG (L-carnitine-genistein). The spot urine samples were collected, and urine protein-to-creatinine ratio was measured. Hepatocytic RNA was extracted and real-time PCR was used for PPAR-alpha and CPT-1 gene Expression measurement. Results: At the end of the study, final weight of the patient groups was considerably lower than the NC group (P=0.001), and weight gain of the NC group was higher than the other groups (P<0.05). The urine protein and urine protein-to-creatinine ratio were significantly lower in LC, G, and LCG groups in comparison with PC group, at week 7 (P<0.001). The expression of PPAR-alpha and CPT-1 mRNA were not significantly higher in LC,G, and LCG groups in comparison with PC group, but Delta CT of these genes showed significant differences between the LC,G, and LCG groups and the PC group (P<0.001). Conclusion: Our study showed an increasing trend in PPAR-alpha and CPT-1 gene expressions, and synergistic effect of L-carnitine and genistein in experimental nephrotic syndrome.