Prevalence and molecular characterization of non-tuberculous mycobacteria in hospital soil and dust of a developing country, Iran

(2019) Prevalence and molecular characterization of non-tuberculous mycobacteria in hospital soil and dust of a developing country, Iran. Microbiology-Sgm. pp. 1306-1314. ISSN 1350-0872

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Official URL: WOS:000522796500005

Abstract

The presence and diversity of mycobacteria that are capable of survival in a harsh and adverse condition, such as hospital environments, have not been comprehensively studied. This study aimed to assess the frequency and diversity of mycobacteria in hospital soil and dust of a developing country using a combination of molecular and conventional methods. A total of 318 hospital dust and soil samples collected from 38 hospitals were analysed using standard protocols for characterization of mycobacteria. The conventional tests were used for preliminary identification and Runyon's classification, the PCR amplification of the hsp65 gene and sequence analyses of 16SrRNA were applied for genus and species identification. In total, 28 samples (8.8) were positive for mycobacteria. The isolates included 33 mycobacteria species including 19 rapidly growing and 14 slowly growing organisms. The most prevalent species were M. setense and M. lentiflavum, five isolates (15.1) each, M. fortuitum, four isolates (12.12) and M. kumamotonense and M. massiliense/abscessus complex three isolates (9.1) each, M. arupense and M. frederiksbergense, two isolates (6) each. The remaining isolates consisted the single strains of eight various mycobacterium species, the results of our study revealed that soil and dust in hospitals can be the reservoir of mycobacteria. This reaffirms the fact that these organisms due to intrinsic resistance can persist in hospitals and create a threat to patient's health, in particular to those who suffer from weakness of immunity.

Item Type: Article
Keywords: non-tuberculous mycobacteria hospital infection 16SrRNA sequencing sp nov. 1st report water identification pulmonary infection disease outbreak avium epidemiology Microbiology
Subjects: QW Microbiology and Immunology > QW 1-300 Microbiology
Divisions: Faculty of Medicine > Department of Basic Science > Department of Microbiology
Page Range: pp. 1306-1314
Journal or Publication Title: Microbiology-Sgm
Journal Index: ISI
Volume: 165
Number: 12
Identification Number: https://doi.org/10.1099/mic.0.000857
ISSN: 1350-0872
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/10971

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