An alarming rise of non-albicans Candida species and uncommon yeasts in the clinical samples; a combination of various molecular techniques for identification of etiologic agents

(2019) An alarming rise of non-albicans Candida species and uncommon yeasts in the clinical samples; a combination of various molecular techniques for identification of etiologic agents. BMC Res Notes. p. 779. ISSN 1756-0500 (Electronic) 1756-0500 (Linking)

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Abstract

OBJECTIVE: Yeasts are unicellular microorganisms may cause systemic infection in immunocompromised patients. The aim of this study was to identify yeast strains isolated from clinical specimens using molecular techniques. RESULTS: A total of 202 yeast strains isolated from 341 clinical samples between February 2017 and May 2019. All clinical isolates were identified using phenotypic and molecular tests including PCR-RFLP, duplex-PCR, multiplex-PCR, and PCR-sequencing. The most yeast fungal isolates were obtained from urine (66.8), nail (9.4), skin lesion (7.9), bronchoalveolar lavage (5.9), and blood (3.9). One hundred and twenty-one Candida species were identified as non-albicans versus 76 Candida albicans. Trichosporon asahii, and Pichia terricola were uncommon non-Candida yeasts isolated from urine samples. For the first time, we isolated P. terricola as etiological agent of urinary tract infection in a pregnant female. Since Candida species show different levels of resistance to antifungal agents, precise identification of clinical isolates is critical for better treatment of infection.

Item Type: Article
Keywords: Adolescent Adult Aged Aged, 80 and over Candida/*genetics/*isolation & purification Candidiasis/microbiology Child Child, Preschool Female Humans Infant Male Middle Aged Molecular Diagnostic Techniques/*methods Polymorphism, Restriction Fragment Length Young Adult Identification Molecular techniques Non-albicans Candida species Pichia terricola Trichosporon asahii Uncommon yeasts
Subjects: WC Communicable Diseases > WC 450-475 Mycoses
Divisions: Faculty of Medicine > Department of Basic Science > Department of Parasitology and Mycology
Infectious Diseases and Tropical Medicine Research Center
Page Range: p. 779
Journal or Publication Title: BMC Res Notes
Journal Index: Pubmed
Volume: 12
Number: 1
Identification Number: https://doi.org/10.1186/s13104-019-4811-1
ISSN: 1756-0500 (Electronic) 1756-0500 (Linking)
Depositing User: Zahra Otroj
URI: http://eprints.mui.ac.ir/id/eprint/11797

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